This will be a research article. I already have the data; but I have been postponing to write it for some time. What I need from you is to write a draft on intestinal crypt organoids and how they utilize different energy sources depending on where they are derived from, the effect of diet on metabolism and cancer, and Western style diet and its effect on colorectal cancer. You can most certainly use the references I gave in my previous published paper (attached). The techniques are similar: crypt isolation and the Seahorse metabolic assays and some more organoid growth assays and proliferation assays will be added. Briefly, I had mouse colonies (Black6) grown as age cohorts (6, 12 and 24months old) grown on three different diets: Western Style Diet, Rescue Diet, and Control Diet. Then I isolated crypts from these mouse (see my attached paper) and cultured in Matrigel until they became crypt organoids. Then, I designed an assay where I checked how these crypt organoids from different sources (mouse on different diets and age groups) utilize alternative energy sources (butyrate, glutamine, glucose). And this is assessed through Seahorse metabolic profiling assays (see the attached paper). I saw a differential utilization of the energy sources with crypts from different mouse cohorts. The additional experiments that were done were crypt growth, where I did not see a difference; apoptosis and cell proliferation assays for crypt organoids, again I did not see a difference. Please, look closely to references at my attached published paper to get a better idea. You can also take a look at the mouse cohorts and the Western style diet related papers.
Overall Structure: A typical research paper with Title, Abstract, Introduction, Materials and Methods, Results, Conclusion and References.
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