1) Outline an efficient strategy to scan a large gene for any mutation. What is the rationale for the main steps in the strategy?
2) Describe the main differences between traditional PCR and real-time PCR.
3) outline the process of screening mutation on large gene
4) describe OLA
5) describe mass spectro
6) Explain how genetic variations are identified by Mass Spectrometry.
7) What is the diagnosing mutation
8) give a forward and reverse primers
5′ …ACGAACTGGCGTA … GGATCGTAAGTCAGT… 3′
3′ …TGCTTGACCGCAT … CCTAGCATTCAGTCA… 5′
(9) describe and compare human genome project, and the different methods used by clera and HGP
(10)the significant/application and use of SNP/hapmap project
(11)what’s alternative splicing, biological significant, briefly describe the types of alternative splicing
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